CHANGES IN BIOCHEMICAL INDICATORS OF BLOOD AND PERIODONTAL TISSUES OF LABORATORY ANIMALS UNDER CONDITIONS OF EXPERIMENTAL HYPOXIA

Abstract

Experimental studies are a key tool for revealing the deep mechanisms of etiopathogenesis of human diseases, which allows to scientifically substantiate the feasibility and safety of further clinical studies. Hypoxia and oxidative stress are the main pathogenetic processes in the development of periodontal tissue diseases. Aim. To determine changes in biochemical parameters of blood and periodontal tissues in laboratory animals under conditions of experimental hypoxia. Materials and methods. To create experimental conditions of hypoxia, a model of iron deficiency anemia was chosen. In 14 white Wistar rats, the following biochemical methods were used to determine: blood condition, MDA, catalase, urease, elastase, lysozyme, dysbiosis, lactate, pyruvate. The degree of alveolar process atrophy was determined by the morphometric method. Statistical analysis. Results. According to the results of the blood test revealed a decrease in the number of erythrocytes by 28.6% (p<0.05) and leukocytes by 77% (p<0.05). According to the results of periodontal tissue studies were found: a decrease in catalase by 11% (p<0.05); an increase in elastase by 28% (p<0.05) and urease by 52% (p<0.05); a decrease in the L/P index by 52% (p<0.01). The degree of alveolar bone atrophy increased by 8.3% (*p<0.05). Conclusions. Experimental hypoxia causes significant changes in the rat organism and periodontal tissues: oxidative stress, reduction of the antioxidant system, development of inflammation, increased bone resorption. The results obtained may serve as a basis for further experimental and clinical studies using drugs to increase the activity of the antioxidant defense system, reduce oxidative stress, activate osteoinduction and correct hypoxia in periodontal tissues in the treatment of periodontal and peri-implant diseases