EVALUATION OF THE EFFICACY OF DIFFERENT THERAPEUTIC APPROACHES FOR THE CORRECTION OF SCAR DEFORMITIES BASED ON MORPHOLOGICAL ANALYSIS OF THE REGENERATED TISSUE IN VIVO

Abstract

The aim of this study was to analyse and evaluate the effectiveness of different methods of correcting soft tissue scar deformities in vivo. Methods of the study.The experimental part of the study was performed on 160 white Wistar rats and consisted of two stages.In the first stage of the experiment, a soft tissue defect was modelled on a pre-prepared back area, and in the second stage, scar deformities were corrected with further study of morphological features at different study periods (7, 14, 30, 60 days). The experiment animals were divided into three groups: Group I – animals in which the correction of scar deformities was performed using the drug “Nascar”; Group II – animals in which the correction of scar deformities was performed using the combination of “Nascar” + CO2 laser; Group III – animals in which the correction of scar deformities was performed using the complex technique of “Nascar” + CO2 laser + PRP microneedling. The histological sections were stained by Van Gieson’s method, haematoxylin-eosin and resorcin-fuchsin by Weigert’s method. The specimens were examined using a Carl Zeiss Jena NU-2 microscope. Scientific novelty. The study found that the use of the drug “Nascar” (group I) provided active regeneration in the early stages, but later the formation of a typical scar was observed. The combination of “Nascar” + CO2 laser (group II) contributed to an improved collagen fibre structure, vascularisation and angiogenesis, resulting in a regeneration similar to normal skin. However, the complex technique involving the use of “Nascar” + CO2 laser + PRP microneedling (group III) showed the most pronounced recovery processes, providing the fastest formation of granulation tissue and a high level of collagen fibre remodelling. Conclusions. Thus, the most effective was the complex approach using “Nascar” + CO2 laser + PRP microneedling, which allowed a rapid and almost complete restoration of the skin structure, reducing scarring and improving the quality of the regenerate.